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There are important differences between quantitative and qualitative de-signs regarding the ways they counter threats to validity. Because the frequency of Tk-deficient cells isvery low (on the order of 1 ? 10?9) in a population of Tk+/+cells, a very large number of cells were required to obtain asmall number of Tk?/? clones

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Because the frequency of Tk-deficient cells isvery low (on the order of 1 ? 10?9) in a population of Tk+/+cells, a very large number of cells were required to obtain asmall number of Tk?/? clones. Riboflavin phosphate(Flavin mononucleotide: FMN) is formed in other tissuesas well

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